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Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia

Received: 6 October 2017     Accepted: 28 October 2017     Published: 30 November 2017
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Abstract

Tuberculosis is an infectious disease that kills approximately three million people annually worldwide. The emergence of multidrug resistant, extensively drug resistant and lengthy therapy reduces the patient compliance and therefore comprises control strategies. In this study, the leaves of Terminalia ivorensis, Carapa procera, Fagara macrophylla, Anacardium occidentale, Ficus spp. and Drepanoalpha® (a polyherbal medicine to relieve sickle cell anaemia) were extracted with petroleum ether, ethyl acetate and methanol in order to screen potential bioactive compounds in different extracts and to assess their anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis spp. on Lowenstein-Jensen medium using a qualitative approach. The activity was determined as to whether there was growth or not. It was shown that only the methanolic extract displayed a good activity on both strains than the petroleum ether and ethyl acetate extracts. The presence of phytochemicals in plants such as alkaloids, flavonoids, tannins, saponins, anthocyanins and quinones known to be of medicinal importance pointed out a possible source for anti-mycobacterial agents to address the problem of multidrug resistance. The in vitro findings of this study provide a partial support for the use of these plants in the control of various infectious diseases as lead to drug discovery and should be reiterated and recommended for a clinical trial using an animal model.

Published in Journal of Diseases and Medicinal Plants (Volume 3, Issue 5)
DOI 10.11648/j.jdmp.20170305.12
Page(s) 88-96
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Tuberculosis, Lowenstein Jensen, Anti-mycobacterial Activity, Phytochemicals, Medicinal Plants

References
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Cite This Article
  • APA Style

    Gédéon Bongo, Huruma Tuntufye, Koto-te-Nyiwa Ngbolua, Joseph Malakalinga, Tshiama Claudine, et al. (2017). Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia. Journal of Diseases and Medicinal Plants, 3(5), 88-96. https://doi.org/10.11648/j.jdmp.20170305.12

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    ACS Style

    Gédéon Bongo; Huruma Tuntufye; Koto-te-Nyiwa Ngbolua; Joseph Malakalinga; Tshiama Claudine, et al. Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia. J. Dis. Med. Plants 2017, 3(5), 88-96. doi: 10.11648/j.jdmp.20170305.12

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    AMA Style

    Gédéon Bongo, Huruma Tuntufye, Koto-te-Nyiwa Ngbolua, Joseph Malakalinga, Tshiama Claudine, et al. Comparative Anti-mycobacterial Activity on Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia. J Dis Med Plants. 2017;3(5):88-96. doi: 10.11648/j.jdmp.20170305.12

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  • @article{10.11648/j.jdmp.20170305.12,
      author = {Gédéon Bongo and Huruma Tuntufye and Koto-te-Nyiwa Ngbolua and Joseph Malakalinga and Tshiama Claudine and Aaron Pambu and Fabrice Mwanza and Clément Mbadiko and Gisèle Makengo and Jeff Iteku and Dorothée Tshilanda and Pius Mpiana and Théophile Mbemba and Rudovick Kazwala},
      title = {Comparative Anti-mycobacterial Activity on  Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia},
      journal = {Journal of Diseases and Medicinal Plants},
      volume = {3},
      number = {5},
      pages = {88-96},
      doi = {10.11648/j.jdmp.20170305.12},
      url = {https://doi.org/10.11648/j.jdmp.20170305.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20170305.12},
      abstract = {Tuberculosis is an infectious disease that kills approximately three million people annually worldwide. The emergence of multidrug resistant, extensively drug resistant and lengthy therapy reduces the patient compliance and therefore comprises control strategies. In this study, the leaves of Terminalia ivorensis, Carapa procera, Fagara macrophylla, Anacardium occidentale, Ficus spp.  and Drepanoalpha® (a polyherbal medicine to relieve sickle cell anaemia) were extracted with petroleum ether, ethyl acetate and methanol in order to screen potential bioactive compounds in different extracts and to assess their anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis spp. on Lowenstein-Jensen medium using a qualitative approach. The activity was determined as to whether there was growth or not. It was shown that only the methanolic extract displayed a good activity on both strains than the petroleum ether and ethyl acetate extracts. The presence of phytochemicals in plants such as alkaloids, flavonoids, tannins, saponins, anthocyanins and quinones known to be of medicinal importance pointed out a possible source for anti-mycobacterial agents to address the problem of multidrug resistance. The in vitro findings of this study provide a partial support for the use of these plants in the control of various infectious diseases as lead to drug discovery and should be reiterated and recommended for a clinical trial using an animal model.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Comparative Anti-mycobacterial Activity on  Lowenstein-Jensen Slants of Selected Medicinal Plants Used in the Congolese Pharmacopeia
    AU  - Gédéon Bongo
    AU  - Huruma Tuntufye
    AU  - Koto-te-Nyiwa Ngbolua
    AU  - Joseph Malakalinga
    AU  - Tshiama Claudine
    AU  - Aaron Pambu
    AU  - Fabrice Mwanza
    AU  - Clément Mbadiko
    AU  - Gisèle Makengo
    AU  - Jeff Iteku
    AU  - Dorothée Tshilanda
    AU  - Pius Mpiana
    AU  - Théophile Mbemba
    AU  - Rudovick Kazwala
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    PY  - 2017
    N1  - https://doi.org/10.11648/j.jdmp.20170305.12
    DO  - 10.11648/j.jdmp.20170305.12
    T2  - Journal of Diseases and Medicinal Plants
    JF  - Journal of Diseases and Medicinal Plants
    JO  - Journal of Diseases and Medicinal Plants
    SP  - 88
    EP  - 96
    PB  - Science Publishing Group
    SN  - 2469-8210
    UR  - https://doi.org/10.11648/j.jdmp.20170305.12
    AB  - Tuberculosis is an infectious disease that kills approximately three million people annually worldwide. The emergence of multidrug resistant, extensively drug resistant and lengthy therapy reduces the patient compliance and therefore comprises control strategies. In this study, the leaves of Terminalia ivorensis, Carapa procera, Fagara macrophylla, Anacardium occidentale, Ficus spp.  and Drepanoalpha® (a polyherbal medicine to relieve sickle cell anaemia) were extracted with petroleum ether, ethyl acetate and methanol in order to screen potential bioactive compounds in different extracts and to assess their anti-mycobacterial activity against Mycobacterium tuberculosis H37Rv and Mycobacterium tuberculosis spp. on Lowenstein-Jensen medium using a qualitative approach. The activity was determined as to whether there was growth or not. It was shown that only the methanolic extract displayed a good activity on both strains than the petroleum ether and ethyl acetate extracts. The presence of phytochemicals in plants such as alkaloids, flavonoids, tannins, saponins, anthocyanins and quinones known to be of medicinal importance pointed out a possible source for anti-mycobacterial agents to address the problem of multidrug resistance. The in vitro findings of this study provide a partial support for the use of these plants in the control of various infectious diseases as lead to drug discovery and should be reiterated and recommended for a clinical trial using an animal model.
    VL  - 3
    IS  - 5
    ER  - 

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Author Information
  • Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo

  • Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania

  • Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo

  • Department of Veterinary Microbiology, Parasitology and Immunology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania

  • Enseignement et Administration en Soins Infirmiers, Section Sciences Infirmières, Institut Supérieur des Techniques Médicales, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo

  • Department of Chemistry, Faculty of Science, Kinshasa, Democratic Republic of the Congo

  • Department of Chemistry, Faculty of Science, Kinshasa, Democratic Republic of the Congo

  • Department of Biology, Faculty of Science, Kinshasa, Democratic Republic of the Congo

  • Department of Veterinary Medicine and Public Health, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania

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