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A Sodium Selective Electrochemical Biosensor Based on the Sodium Pump

Received: 19 July 2016     Published: 20 July 2016
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Abstract

Na+ is one of the most abundant metal ions and plays critical physiological roles in biological systems, therefore development of new Na+ sensors is becoming increasingly important. Currently many physical and chemical methods have been applied to detect Na+. In this paper, we developed a novel Na+ electrode based on a biomaterial, light-driven Na+ pump (NdR2). The photocurrent of the electrode upon excitation shows a characteristic positive polarity in Na+ solution at neutral pH. The peak current shows Na+ dependency within the concentration range from 1 to 200 mmol/L. The stability data show that the electrode is stable after being stored at 50°C for 90 min. As a novel biosensor, this Na+ pump-based electrode is of great significance in the study of ion transport mechanism and application of ion detection.

Published in Science Discovery (Volume 4, Issue 4)
DOI 10.11648/j.sd.20160404.16
Page(s) 238-242
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

NdR2, NdR2-liposome-ITO Electrode, Na+ Concentration, Biosensor

References
[1] Malloy, C. R. et al. Influence of Global-Ischemia on Intracellular Sodium in the Perfused Rat-Heart [J]. Magnet Reson Med, 1990, 15:33-44.
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[3] Ivanics, T., Blum, H., Wroblewski, K., Wang, D. J. & Osbakken, M. Intracellular Sodium in Cardiomyocytes Using Na-23 Nuclear-Magnetic-Resonance [J]. Bba-Mol Cell Res, 1994, 1221:133-144.
[4] Torabi, S. F. et al. In vitro selection of a sodium-specific DNAzyme and its application in intracellular sensing [J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112:5903-5908.
[5] Oesterhelt, D. & Stoeckenius, W. Rhodopsin-like protein from the purple membrane of Halobacterium halobium [J]. Nature: New biology, 1971, 233:149-152.
[6] Liu, S. Y. & Ebrey, T. G. Photocurrent measurements of the purple membrane oriented in a polyacrylamide gel [J]. Biophys J,1988, 54:321-329.
[7] Cao, Y. et al. Water is required for proton transfer from aspartate-96 to the bacteriorhodopsin Schiff base [J]. Biochemistry, 1991, 30:10972-10979.
[8] Bertoncello, P., Nicolini, D., Paternolli, C., Bavastrello, V. & Nicolini, C. Bacteriorhodopsin-based Langmuir-Schaefer films for solar energy capture [J]. IEEE transactions on nanobioscience, 2003, 2:124-132.
[9] Hong, F. T. Molecular Sensors based on the photovoltaic effect of bacteriorhodopsin: origin of differential responsivity [J]. Materials Science and Engineering, 1997.
[10] Miyasaka, T., Koyama, K. & Itoh, I. Quantum conversion and image detection by a bacteriorhodopsin-based artificial photoreceptor [J]. Science, 1992, 255:342-344.
[11] Choi, H. G., Jung, W. C., Min, J. H., Lee, W. H. & Choi, J. W. Color image detection by biomolecular photoreceptor using bacteriorhodopsin-based complex LB films [J]. Biosensors & bioelectronics, 2001, 16:925-935.
[12] Ahmadi, M. & Yeow, J. T. Fabrication and characterization of a radiation sensor based on bacteriorhodopsin [J]. Biosensors & bioelectronics, 2001, 26:2171-2176.
[13] Rao, S. et al. A proteorhodopsin-based biohybrid light-powering pH sensor [J]. Physical chemistry chemical physics, 2013, 15:15821-15824.
[14] Rao, S. et al. A light-powered bio-capacitor with nanochannel modulation [J]. Advanced materials, 2014, 26:5846-5850.
[15] Kwon, S. K. et al. Genomic makeup of the marine flavobacterium Nonlabens (Donghaeana) dokdonensis and identification of a novel class of rhodopsins [J]. Genome biology and evolution, 2013, 5:187-199.
[16] Inoue, K. et al. A light-driven sodium ion pump in marine bacteria [J]. Nature communications, 2013, 4:1678.
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Cite This Article
  • APA Style

    Li Longjie, Zhao Hongshen, Ji Liangliang, Ma Baofu, Chen Deliang. (2016). A Sodium Selective Electrochemical Biosensor Based on the Sodium Pump. Science Discovery, 4(4), 238-242. https://doi.org/10.11648/j.sd.20160404.16

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    ACS Style

    Li Longjie; Zhao Hongshen; Ji Liangliang; Ma Baofu; Chen Deliang. A Sodium Selective Electrochemical Biosensor Based on the Sodium Pump. Sci. Discov. 2016, 4(4), 238-242. doi: 10.11648/j.sd.20160404.16

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    AMA Style

    Li Longjie, Zhao Hongshen, Ji Liangliang, Ma Baofu, Chen Deliang. A Sodium Selective Electrochemical Biosensor Based on the Sodium Pump. Sci Discov. 2016;4(4):238-242. doi: 10.11648/j.sd.20160404.16

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  • @article{10.11648/j.sd.20160404.16,
      author = {Li Longjie and Zhao Hongshen and Ji Liangliang and Ma Baofu and Chen Deliang},
      title = {A Sodium Selective Electrochemical Biosensor Based on the Sodium Pump},
      journal = {Science Discovery},
      volume = {4},
      number = {4},
      pages = {238-242},
      doi = {10.11648/j.sd.20160404.16},
      url = {https://doi.org/10.11648/j.sd.20160404.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20160404.16},
      abstract = {Na+ is one of the most abundant metal ions and plays critical physiological roles in biological systems, therefore development of new Na+ sensors is becoming increasingly important. Currently many physical and chemical methods have been applied to detect Na+. In this paper, we developed a novel Na+ electrode based on a biomaterial, light-driven Na+ pump (NdR2). The photocurrent of the electrode upon excitation shows a characteristic positive polarity in Na+ solution at neutral pH. The peak current shows Na+ dependency within the concentration range from 1 to 200 mmol/L. The stability data show that the electrode is stable after being stored at 50°C for 90 min. As a novel biosensor, this Na+ pump-based electrode is of great significance in the study of ion transport mechanism and application of ion detection.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - A Sodium Selective Electrochemical Biosensor Based on the Sodium Pump
    AU  - Li Longjie
    AU  - Zhao Hongshen
    AU  - Ji Liangliang
    AU  - Ma Baofu
    AU  - Chen Deliang
    Y1  - 2016/07/20
    PY  - 2016
    N1  - https://doi.org/10.11648/j.sd.20160404.16
    DO  - 10.11648/j.sd.20160404.16
    T2  - Science Discovery
    JF  - Science Discovery
    JO  - Science Discovery
    SP  - 238
    EP  - 242
    PB  - Science Publishing Group
    SN  - 2331-0650
    UR  - https://doi.org/10.11648/j.sd.20160404.16
    AB  - Na+ is one of the most abundant metal ions and plays critical physiological roles in biological systems, therefore development of new Na+ sensors is becoming increasingly important. Currently many physical and chemical methods have been applied to detect Na+. In this paper, we developed a novel Na+ electrode based on a biomaterial, light-driven Na+ pump (NdR2). The photocurrent of the electrode upon excitation shows a characteristic positive polarity in Na+ solution at neutral pH. The peak current shows Na+ dependency within the concentration range from 1 to 200 mmol/L. The stability data show that the electrode is stable after being stored at 50°C for 90 min. As a novel biosensor, this Na+ pump-based electrode is of great significance in the study of ion transport mechanism and application of ion detection.
    VL  - 4
    IS  - 4
    ER  - 

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Author Information
  • College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China

  • College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China

  • College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China

  • College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China

  • College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China

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